指導論文
論文 標題 | Isolation and identification of acetic acid bacteria isolates based on molecular techniques (利用分子生物技術進行醋酸菌篩選及鑑定之研究) |
研究生 | Min-hsiu Chang(張敏修) |
指導 教授 | Shu-jen Wang(王淑珍)、Jiau-hua Chen(陳椒華) |
摘要 | In this study, primers were designed on the basis of the available acetic acid bacteria (AAB) type III alcohol dehydrogenase gene (adh ) (acc. no. AB264314) sequence . The specificity of this set of primers ADH III L187 / ADH III R500 was tested with well defined strains of AAB and non AAB strains, such as Bacillus cereus, Yersinia enterocolitica, Salmonella enteritidis D1, Lactobacillus amylovorus, Pseudomonas aeruginosa and nont6oxigenic E.coli. From the results, the specificity of this set of primers ADH III L187 / ADH III R500 was confirmed. This set of primers was further used for detection of 79 AAB isolated strains directly from different sources. The results showed that 41 isolated strains tested successfully with the same size of PCR products as reference strains of acetic acid bacteria. During the test of acidity, 29 isolated strains were selected for their ability of growth and high acetate production in YGM medium. Identification of these 29 isolated strains were done by analysis 16S rDNA sequence and the isolated strains were clustered. All tested isolated strains were belonged to Acetobacter spp.. Further study evaluated the alcohol tolerance of AAB isolates. The results showed that AAB305 strains was able to endure 7 % alcohol in YGM medium. In the future, this strain will be used in vinegar fermentation. |
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