論文發表

論文發表

我的實驗室工作主要是大學畢業後考上清華大學研究所，然後到食品工業發展研究所進行論文，畢業後則繼續於該所工作。

三十多年前食品所的微生物實驗室是國內少數進行生物技術實驗的實驗室，我的論文是進行細菌基因選殖，指導教授是許文輝博士，他主持了食品所的菌種中心。我的論文及研究範疇技術有包括DNA、蛋白質、免疫分析、PCR、微生物檢驗等。

論文名稱	出版 日期	期刊名稱	期頁數	技術
Antibacterial activity Lactobacillus plantarum isolated from fermented vegetables and investigation of the plantaricin genes	2016	African Journal of Microbiology Research	Vol22	抗菌性分析
雲芝菌絲體發酵液凍乾粉之安全性探討	2016	澄  清  醫  護  管  理  雜  誌	Vol6	發酵、安全性分析
Beneficial Effect of Sugar Osmolytes on the Refolding of Guanidine Hydrochloride-Denatured Trehalose-6-phosphate Hydrolase from Bacillus licheniformis	2014	BioMed Research International		蛋白質分析
A Rapid and Specific PCR Method for the Detection of Shigella spp. in Spiked Samples	2012	Journal of Food and Drug Analysis	Vol20	PCR方法
Enzymatic characterization of Bacillus licheniformis [1]-glutamyltranspeptidase	2012	Enzyme and Microbial Technology 51 (2012) 86– 94	Vol51	蛋白質
Unfolding analysis of the mature and unprocessed forms of Bacillus licheniformis γ-glutamyltranspeptidase	2011	Journal of Biological Physics	Vol37	蛋白質
Mutational Analysis of Splicing Activities of Ribonucleotide Reductase a Subunit Protein from Lytic Bacteriophage P1201	2011.1	Curr Microbiol	Vol62   P1282–1286	蛋白質
良性攝護腺肥大(Benign Prostatic Hyperplasia, BPH)生物性病因的探討	2010.12	嘉南學報(科技類)	Vol36   p.65-71	DNA
Site-Saturation Mutagenesis of Leucine 134 of Bacillus	2010.06	Protein J (2010) 29:365–372	Vol.29	DAN
Preparation of carboxylated magnetic particles for the eYcient	2010.01	J Ind Microbiol Biotechnol (2010) 37:717–725	Vol.37	蛋白質
應用聚合酶鏈反應(PCR)技術鑑定分離自傳統家庭釀造液之醋酸菌(Acetobacter sp.)	2009.12	嘉南學報	NO.35	PCR
有效微生物之分離與除臭試驗	2009.12	嘉南學報	NO.35
A Study on Comparison of University Biology Text	2008.12	嘉南學報（人文類）	NO.34    Vol.1
牛羊體表毒素形及非毒素型葡萄球菌之分佈.	2002.10	中國畜產學會會誌	NO.31    Vol.1	免疫分析 微生物
超市半調理食品與不同方法自製半調理食品儲存期間微生物之變化	1999.12	藥物食品分析	NO.4    Vol..7	免疫分析 微生物
台南地區養殖魚塭 、生鮮超市及傳統零售市場吳郭魚及虱目魚之微生物調查	1996.12	藥物食品分析	NO.4    Vol.4	免疫分析 微生物
梅納褐變反應產物誘導DNA變異之研究	1996.03	藥物食品分析	NO. 1  Vol.4	DNA
測定甜味劑斯巴甜之酵素感測器	1995.07	藥物食品分析	NO. 2  Vol.3	酵素
新鮮吳郭魚及虱目魚在冷藏(4℃)及凍藏(-15℃)期間品質之變化	1994.12	藥物食品分析	NO. 4  Vol.2	免疫分析 微生物
Cloning and characterization of an α-amylase gene from Streptomyces lividans	1993.01	Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression	Issue 3    Volume 1171	DNA
Schwanniomyces occidentalis 基因選殖系統建立之研究	1992.11	嘉南學報	NO.18    Vol.18	DNA
Pseudomonas amyloderamosa 澱粉枝切酶基因選殖	1990.12	中國農業化學會誌	NO.4  Vol.28	DNA
Expression of Isoamylase Gene from Pseudomonas amy lideramosa JD210	1990.10	Peoc. Natl. Sci. B.ROC	NO.4    Vol.14	DNA 蛋白質
Nucletide sequence and expression of the isoamylase gene from an isoamylase- hyperproducing mutant,Pseudomonas amyloderamosa  JD210	1990.06	Biochimice et Biophvsica Acta,	1087	DNA

 

研討會論文

 研討會論文

論文名稱	所屬計畫	研討會名稱	年度
Antioxidant effects of isolated lactic acid bacteria and protective role in DNA	奇美醫院合作計畫	ISNFF(Nutraceutical, Functional Foods, Natural Health Products and Dietary Supplements)	2008
以不同菌種生產奈米化桑椹醋品質之研究	無	第三十七次食品科學技術學會	2007
由傳統釀造醋進行醋酸菌之分離與鑑定分析	嘉藥計畫	第三十六次食品科學技術學會	2006
肉雞飼料用乳酸菌株之篩選分離	無	中國畜牧學會會誌35(增刊)	2006
發展並應用高特異性type III alcohol dehydrogenase gene’s primer快速檢測醋酸菌.	無	第三十六次食品科學技術學會	2006
有益微生物於廚餘堆肥化之應用	嘉藥計畫	第三十六次食品科學技術學會	2006
醋酸菌之發酵試驗與發酵產物分析	嘉藥計畫	第三十六次食品科學技術學會	2006
短期作物小白菜與青江菜施以廚餘有機肥、大豆有機肥及化學肥之栽種成份	嘉藥計畫	第三十四次台灣食品科學技術年會	2004
有益微生物乳酸菌之分離與鑑定	嘉藥計畫	第三十三次台灣食品科學技術年會	2003
葡萄酒生產菌之篩選、發酵及鑑定試驗	嘉藥計畫	第三十三次台灣食品科學技術年會	2003
保育團體結合社區產業的生態旅遊模式—以七股為例	無	無	2003
“Experiences in Developing an Organic Kitchen Waste Recycling System in Tainan Area, Taiwan.”	無	無	2003
良性攝護腺肥大生物性病因之研究	奇美醫院研究計畫	第十八屆臺灣外科醫學會	2002
環保義工參與社區學校「廚餘回收再利用」宣導成功案例	無	無	2002
Isolation and Characterization of a Photosynthetic Bacterium, Rhodobacter JH413	無	American Society for Microbiology, 102 nd General Meeting,	2002
Designing of polymerase chain reaction primers for the detection of Salmonella enteriditis in foods and fecal samples.	無	第四十屆中國農業化學會	2002

指導論文_鄰近於高壓輸配電線民眾之健康情形研究_以中部某社區為例(2009)

Study on the Health of People Living in the Vinicity of High Voltage Transmission Lines -An Example of A Community in Central Taiwan

(鄰近於高壓輸配電線民眾之健康情形研究

-以中部某社區為例)

Tzu-Chen Chang (張子見),Shu-Hua Chen (陳椒華), Chun-Liang Tu (塗鈞亮)

中文關鍵詞：非游離輻射、極低頻電磁輻射、高壓電纜、自覺症狀

English Keyword：Non-Ionizing Radiation、Extremely Low Frequency-Electromagnetic field、High Voltage Transmission Lines、Subjective Symptoms

Link：

http://ndltd.ncl.edu.tw/cgi-bin/gs32/gsweb.cgi/ccd=FsEgUp/record?r1=1&h1=0

Abstract：

The main purpose of this study was to investigate the strength of residential indoor electromagnetic field of the high-voltage distribution lines on both sides.  The high voltage distribution lines and the distance to the area were divided into experimental and control groups. In Taichung County's Wufeng Township, the magnetic filed strength of the household living room and bed room were mearused every two minutes for 24 hours, calculating the Minimum, Maximum, Arithmetic average, Standard deviation and the weighted average living room and bedroom.  At the meantime, adopte a questionnaire survey to find out the health situation of the households.  By analysising the data and result from the survey to indect the association between the magnetic field intensity on the health status of residents.
The results show that the magnetic field strength averaged 4.46±5.67 mG within the 69 household received the mearusement, experimental group, the average magnetic field （7.93±6.87mG）is higher than the average magnetic field (1.45±0.33 mG).  The result shows that the shorter distance from the high-voltage distribution lines recently, the higher magnetic field strength.
Its two groups of variables with symptoms have a very significant difference, negative correlation faxianjuyou negative correlation exists, the nearer subjective symptoms and health were on the yingxiang are greater. Post hoc comparison showed that the respondents by age, it affected the subjective symptoms, but the incidence raises to the same age comparing with the experimental group were higher.  This indicats the impact of distance for the subjective symptoms is higher than the age factor.
In addition divid the magnetic field into less than 3mG ,3-8 mG, and more than 8mG three grades, the resoult showed different magnetic field strength has significant difference to the awareness of subjective symptoms.  Thec omparsion of the effect of respondents’ subjective symptoms within the same age, magnetic field intensity higher than 3 mG has also got on higher consciousness of the impact of subjective symptoms.

本研究主要目的為調查高壓輸配電線兩側住家室內電磁場強度，並進一步比較電磁場強度所致的健康情形；並以距離高壓輸配電線的遠近區分為實驗組及對照組。於台中縣霧峰鄉甲寅村高壓輸配電線路兩側之住戶，分別量測該住戶客廳及臥室磁場強度，每兩分鐘紀錄瞬間磁場強度共量測24小時，計算其最小值、最大值、算數平均值、標準差及客廳與臥室的加權平均；並利用問卷調查方式，得知該戶居民之健康情形，分析磁場強度對居民健康狀況之關聯性。
研究結果顯示，共測得69戶，磁場強度平均值為4.46±5.67 mG，實驗組磁場平均值（7.93±6.87mG）明顯高於對照組磁場平均值(1.45±0.33 mG)，顯示距離高壓輸配電線愈近者，其磁場強度愈高。其兩組與自覺症狀變項上有極顯著性的差異，且呈現負相關存在，顯示距離愈靠近者對自覺症狀及健康的影響也愈大。經事後比較發現，受訪者自覺症狀故受年齡影響，但就同一年齡層的罹患率來比對，實驗組皆高於對照組，顯示距離對於自覺症狀的影響高於年齡因素。
此外將磁場強度區分為小於3mG、3-8 mG及大於8mG三個等級，結果顯示不同的磁場強度，對自覺性症狀有極顯著性差異，經事後比較發現，受訪者對自覺症狀影響就同一年齡層比較，磁場強度高於3 mG者對自覺性症狀的影響也愈高。

指導論文_利用分子生物技術進行醋酸菌篩選及鑑定之研究(2007)

指導論文

論文 標題	Isolation and identification of acetic acid bacteria isolates based on molecular techniques (利用分子生物技術進行醋酸菌篩選及鑑定之研究)
研究生	Min-hsiu Chang(張敏修)
指導 教授	Shu-jen Wang(王淑珍)、Jiau-hua Chen(陳椒華)
摘要	In this study, primers were designed on the basis of the available acetic acid bacteria (AAB) type III alcohol dehydrogenase gene (adh ) (acc. no. AB264314) sequence . The specificity of this set of primers ADH III L187 / ADH III R500 was tested with well defined strains of AAB and non AAB strains, such as Bacillus cereus, Yersinia enterocolitica, Salmonella enteritidis D1, Lactobacillus amylovorus, Pseudomonas aeruginosa and nont6oxigenic E.coli. From the results, the specificity of this set of primers ADH III L187 / ADH III R500 was confirmed. This set of primers was further used for detection of 79 AAB isolated strains directly from different sources. The results showed that 41 isolated strains tested successfully with the same size of PCR products as reference strains of acetic acid bacteria. During the test of acidity, 29 isolated strains were selected for their ability of growth and high acetate production in YGM medium.     Identification of these 29 isolated strains were done by analysis 16S rDNA sequence and the isolated strains were clustered.  All tested isolated strains were belonged to Acetobacter spp.. Further study evaluated the alcohol tolerance of AAB isolates. The results showed that AAB305 strains was able to endure 7 % alcohol in YGM medium. In the future, this strain will be used in vinegar fermentation.
連結	http://ndltd.ncl.edu.tw/cgi-bin/gs32/gsweb.cgi/ccd=2er2jl/record?r1=2&h1=3